ANTIBODY ENGINEERING
Protein Expression
Gene synthesis, vector construction.
Transient expression 6-8 weeks, 0-500 mg proteins.
Antibody / Bispecific Antibody / Fusion protein / Antigen.
Antibody Humanization
Start with sequence of the mouse-origin monoclonal antibody, design, construct, screening.
Short time (2 months) and higher binding affinity.
Antibody Fc Engineering
ADCC increase / weaken
CDC increase / weaken
ADCP increase / weaken
FcRn binding increase / weaken
Antibody design for ADC
CHEMICAL SYNTHESIS
Design and Synthesis of Linker-Payloads
- A library of ready-for-use Linker-Payloads for rapid ADC screening.
- Design and synthesis of customized Linker-Payloads.
- Synthesis process development of Linker-Payloads and related precursors.
- Milligram to gram scale supply of Linker-Payloads and precursors.
ANTIBODY DRUG CONJUGATION SCREENING
ADC Conjugation Service
- Lysine-based conjugation
- Cysteine-based conjugation (Single-thiol conjugation /Double-thiol covalent conjugation)
- Site-specific conjugation
- For different types of antibody (IgG1/ IgG2 / IgG4 / Bi-specific antibody)
- For different kinds of Linker- Payloads
ADC Assay and Mechanism Study
ADC analysis (For early stage)
- DAR / Free drug ratio / Concentration / Purity etc.
ADC Mechanism Study (For early stage)
- Tumor inhibition potency evaluation assay
- Binding affinity activity assay
- Antigen expression level detection
- Endocytosis activity assay
- Stability in serum assay
- Animal experiment (Tumor Models /MTD/PK/PD— mouse/rat)
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